- Sic1 Mutants
- sic1∆
- sic1∆ cdh1∆
- sic1∆ cdh1∆ GALL-CDC20
- sic1∆ cdc6∆2-49
- sic1∆ cdc6∆2-49 cdh1∆
- sic1∆ cdc6∆2-49 cdh1∆ GALL-CDC20
- GAL-CLB2 sic1∆
- GAL-CLB5 sic1∆
- CLB5-db∆ sic1∆
- APC-A sic1∆
- APC-A sic1∆ cdc6∆2-49
- cdc14-ts sic1∆
- cln1∆ cln2∆ sic1∆
- cln3∆ bck2∆ sic1∆
- cln1∆ cln2∆ cln3∆ sic1∆
- swi5∆
- swi5∆ GAL-CLB2
- swi5∆ cdh1∆
- swi5∆ cdh1∆ GAL-SIC1
- GAL-SIC1
- GAL-SIC1-db∆
- GAL-SIC1 cln1∆ cln2∆
- GAL-SIC1 GAL-CLN2 cln1∆ cln2∆
- GAL-SIC1 cln1∆ cln2∆ cdh1∆
- GAL-SIC1 GAL-CLN2 cln1∆ cln2∆ cdh1∆
- CLB2-db∆ multicopy SIC1
- CLB2-db∆ GAL-SIC1
- cdc14-ts GAL-SIC1
- cdc14ts then GAL-SIC1
- APC-A cdh1∆ GAL-SIC1
- APC-A cdh1∆ multi-copy SIC1
CLB2-db∆ multicopy SIC1
debug: ,
test user =
test db =
Simulation:
Change of parameters: kdb2p=0, kdb2"=0.03, ksc1'=0.024, ksc1"=0.24 (=2 copies).
Length of G1 phase: 53 min.
Mass at division: 2.94.
Experiments:
Cross, F.R. (2003). Two redundant oscillatory mechanisms in the yeast cell cycle. Dev. Cell 4:741-752.
[Abstract] [Article]
[Abstract] [Article]
Experimental results: Supplementary Fig. 5. Low copy number (CEN) plasmids containing SIC1 gene controlled by its own promoter result in partial rescue of the inviability of CLB2-db∆ when grown in glucose medium, whereas high copy number (2 μ) plasmids containing
SIC1 gene show strong rescue.
Comments:
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